Detail Information
P450 Protein:
| P450 Symbol | CYP102A1 |
| Protein Name | Bifunctional cytochrome P450/NADPH--P450 reductase |
| Uniprot ID | P14779 |
| EC Number | 1.14.14.1; 1.6.2.4 |
| Gene ID | 93643681 |
| Species | Bacillus megaterium (strain ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / NRRL B-14308 / VKM B-512) |
| Txid | 1348623 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C16H32O2 → C16H32O3 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Solvents | Dimethyl sulfoxide;Water |
| Conditions | 12 h, pH 8, 30°C |
CYP102A1
Substrate Information:
| Substrate Name | hexadecanoic acid |
| Substrate Chemical Formula | C16H32O2 |
| Substrate PubChem CID | 985 |
| Substrate Smiles | CCCCCCCCCCCCCCCC(=O)O |
| Substrate Structure |
Product Information:
| Product Name | 16-hydroxyhexadecanoic acid |
| Product Chemical Formula | C16H32O3 |
| Product PubChem CID | 10466 |
| Product CAS Number | 506-13-8 |
| Product Smiles | C(CCCCCCCC(=O)O)CCCCCCCO |
| Product Structure |
References:
| Title: | High Throughput Screening Method for Engineering P450 Towards Terminal Hydroxylation of Fatty Acids |
| DOI: | https://doi.org/10.1166/jbmb.2019.1816 |
| Journal: | Journal of Biobased Materials and Bioenergy |
| Year: | 2019/2/1 |