Detail Information
P450 Protein:
| P450 Symbol | |
| Protein Name | p450 |
| Uniprot ID | |
| Species | Streptomyces hygroscopicus subsp. hygroscopicus (DSM-40578) |
| Txid | 1912 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C24H40O4 → C24H40O5 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Solvents | Water |
| Conditions | 18 h, pH 8.5, 24°C |
| Transformations | Hydroxylation at an Aliphatic Carbon |
Substrate Information:
| Substrate Name | (4R)-4-[(3R,5R,8R,9S,10S,12S,13R,14S,17R)-3,12-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoic acid |
| Substrate Chemical Formula | C24H40O4 |
| Substrate PubChem CID | 222528 |
| Substrate Smiles | C[C@H](CCC(=O)O)[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2CC[C@H]4[C@@]3(CC[C@H](C4)O)C)O)C |
| Substrate Structure |
Product Information:
| Product Name | (4R)-4-[(3R,5S,7S,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoic acid |
| Product Chemical Formula | C24H40O5 |
| Product PubChem CID | 122340 |
| Product CAS Number | 2955-27-3 |
| Product Smiles | C[C@H](CCC(=O)O)[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C |
| Product Structure |
References:
| Title: | Method for Hydroxylation of Steroids |
| Patent ID: | EP3875596 |
| Journal: | |
| Year: | 2021/9/8 |