Detail Information

P450 Protein:

P450 Symbol
Protein NameP450DA-M3
Uniprot ID
SpeciesDeinococcus aerius
Txid 200253
P450 Protein Structure
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
Powered by PDBe Molstar & AlphaFold
Subcellular Location
Protein Sequence GO

Reaction Scheme:

Chemical Conversion C9H11Br → C9H11BrO
Reaction TypeOxidation
Chemical BondCH
SolventsEthanol;Water
Conditions24 h, pH 8.5, 30°C
TransformationsHydroxylation at an Aliphatic Carbon

1-(2-bromoethyl)-3-methylbenzene

(1S)-2-bromo-1-(3-methylphenyl)ethanol

Substrate Information:

Substrate Name 1-(2-bromoethyl)-3-methylbenzene
Substrate Chemical FormulaC9H11Br
Substrate PubChem CID570684
Substrate SmilesCC1=CC(=CC=C1)CCBr
Substrate Structure

Product Information:

Product Name (1S)-2-bromo-1-(3-methylphenyl)ethanol
Product Chemical FormulaC9H11BrO
Product PubChem CID141407515
Product CAS Number1817798-19-8
Product SmilesCC1=CC(=CC=C1)[C@@H](CBr)O
Product Structure

References:

Title: Directed evolution of cytochrome P450DA hydroxylase activity for stereoselective biohydroxylation
DOI: https://doi.org/10.1039/D2CY00164K
Journal: Catalysis Science & Technology
Year: 2022/8/11

Contact zhy1001@alu.uestc.edu.cn or yangzhang@cdutcm.edu.cn
© Department of Bioinformatics