Detail Information
P450 Protein:
| P450 Symbol | CYP1251 C3 |
| Protein Name | Cytochrome P450Blt |
| Uniprot ID | A0ACD6BAH8 |
| EC Number | 1.14.-.- |
| Species | Micromonospora sp. MW-13 |
| Txid | 2094022 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C32H50N10O7S → C32H48N10O8S |
| Reaction Type | Cyclization |
| Chemical Bond | |
| Solvents | Water |
| Conditions | overnight, 30°C |
CYP1251 C3
Substrate Information:
| Substrate Name | (2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoyl]amino]-3-(1H-imidazol-5-yl)propanoic acid |
| Substrate Chemical Formula | C32H50N10O7S |
| Substrate PubChem CID | 169501713 |
| Substrate Smiles | [C@@H](CC1=CC=C(O)C=C1)(NC([C@@H](NC([C@H](CCSC)N)=O)CCCNC(=N)N)=O)C(N[C@H](C(N[C@@H](CC2=CN=CN2)C(O)=O)=O)CC(C)C)=O |
| Substrate Structure |
Product Information:
| Product Name | (7S,10S,13S)-13-{[(2S)-2-{[(2S)-2-amino-4-[methyl(oxo)-lambda4-sulfanyl]-1-oxobutyl]amino}-5-{[amino(azanylidene)methyl]amino}-1-oxopentyl]amino}-18-hydroxy-10-(2-methylpropyl)-9,12-dioxo-2,4,8,11-tetrazatricyclo[13.3.1.12,5]icosa-1(19),3,5(20),15(16),17-pentaene-7-carboxylic acid |
| Product Chemical Formula | C32H48N10O8S |
| Product CAS Number | 2951913-30-5 |
| Product Smiles | OC1=C2N3C=C(N=C3)C[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](NC([C@@H](NC([C@H](CCS(C)=O)N)=O)CCCNC(=N)N)=O)CC(=C2)C=C1 |
| Product Structure |
References:
| Title: | Cytochrome P450(Blt) Enables Versatile Peptide Cyclisation to Generate Histidine- and Tyrosine-Containing Crosslinked Tripeptide Building Blocks. |
| PMID: | 35851739 |
| Journal: | Angewandte Chemie, International Edition |
| Year: | 2022/7/18 |