Detail Information
P450 Protein:
| P450 Symbol | CYP1251 C3 |
| Protein Name | Cytochrome P450Blt |
| Uniprot ID | A0ACD6BAH8 |
| EC Number | 1.14.-.- |
| Species | Micromonospora sp. MW-13 |
| Txid | 2094022 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C33H45N9O7S → C33H43N9O8S |
| Reaction Type | Cyclization |
| Chemical Bond | |
| Solvents | Water |
| Conditions | overnight, 30°C |
CYP1251 C3
Substrate Information:
| Substrate Name | N-[(5S,8S,11S)-5-amino-8-(3-{[amino(azanylidene)methyl]amino}propyl)-11-[(4-hydroxyphenyl)methyl]-6,9,12,15-tetraoxo-2-thia-7,10,13-triazapentadec-15-yl]-L-tryptophan |
| Substrate Chemical Formula | C33H45N9O7S |
| Substrate Smiles | C([C@H](NC(CNC([C@H](CC1=CC=C(O)C=C1)NC([C@@H](NC([C@H](CCSC)N)=O)CCCNC(=N)N)=O)=O)=O)C(O)=O)C=2C=3C(NC2)=CC=CC3 |
| Substrate Structure |
Product Information:
| Product Name | (13S,19S)-19-{[(2S)-2-{[(2S)-2-amino-4-[methyl(oxo)-lambda4-sulfanyl]-1-oxobutyl]amino}-5-{[amino(azanylidene)methyl]amino}-1-oxopentyl]amino}-15,18-dioxo-2-oxa-4,14,17-triazatetracyclo[19.2.2.03,11.05,10]pentacosa-1(24),3(11),5(6),7,9,21(25),22-heptaene-13-carboxylic acid |
| Product Chemical Formula | C33H43N9O8S |
| Product CAS Number | 2951913-50-9 |
| Product Smiles | C(O)(=O)[C@@H]1CC2=C(NC=3C2=CC=CC3)OC=4C=CC(=CC4)C[C@H](NC([C@@H](NC([C@H](CCS(C)=O)N)=O)CCCNC(=N)N)=O)C(=O)NCC(=O)N1 |
| Product Structure |
References:
| Title: | Cytochrome P450(Blt) Enables Versatile Peptide Cyclisation to Generate Histidine- and Tyrosine-Containing Crosslinked Tripeptide Building Blocks. |
| PMID: | 35851739 |
| Journal: | Angewandte Chemie, International Edition |
| Year: | 2022/7/18 |