Detail Information
P450 Protein:
| P450 Symbol | cyp53A1 |
| Protein Name | Benzoate 4-monooxygenase bphA (EC 1.14.14.92) (Benzoate-para-hydroxylase A) (BpH) (Cytochrome P450 monooxygenase cyp53A1) |
| Uniprot ID | A2QTW5 |
| EC Number | 1.14.14.92 |
| Gene ID | 4983886 |
| Species | Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) |
| Txid | 425011 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C7H5O2- + C17H21N4O9P-2 + O2 → C7H6O3 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 18033 |
+
+
cyp53A1
+
+
+
Substrate Information:
| Substrate Name | benzoate |
| Substrate Chemical Formula | C7H5O2- |
| Substrate PubChem CID | 242 |
| Substrate Smiles | C1=CC=C(C=C1)C(=O)[O-] |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 4-hydroxybenzoate |
| Product Chemical Formula | C7H6O3 |
| Product PubChem CID | 135 |
| Product Smiles | C1=CC(=CC=C1C(=O)O)O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. |
| PMID: | 17259976 |
| Journal: | Molecular & general genetics : MGG |
| Year: | 1991/4/ |
| Title: | Genetic analysis of Aspergillus niger mutants defective in benzoate-4-hydroxylase function. |
| PMID: | 1868576 |
| Journal: | Archives of biochemistry and biophysics |
| Year: | 2000/5/ |
| Title: | Regulation of expression of the Aspergillus niger benzoate para-hydroxylase cytochrome P450 system. |
| PMID: | 10852481 |
| Journal: | Current genetics |
| Year: | 2001/10/15 |
| Title: | Purification and characterization of benzoate-para-hydroxylase, a cytochrome P450 (CYP53A1), from Aspergillus niger. |
| PMID: | 11594739 |
| Journal: | Nature biotechnology |
| Year: | 2007/2/ |