Detail Information
P450 Protein:
| P450 Symbol | CYP726A27 |
| Protein Name | Cytochrome P450 726A27 (ElCYP726A27) (4,5,8-trihydroxycasbene synthase) (EC 1.14.14.-) (4,8-dihydroxycasbene synthase) (EC 1.14.14.-) (4-hydroxycasbene synthase) (EC 1.14.14.-) |
| Uniprot ID | A0A161GJD5 |
| EC Number | 1.14.14.- |
| Species | Euphorbia lathyris (Caper spurge) |
| Txid | 212925 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C20H32 + C17H21N4O9P-2 + O2 → C20H32O + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 65588 |
+
+
CYP726A27
+
+
+
Substrate Information:
| Substrate Name | (-)-casbene |
| Substrate Chemical Formula | C20H32 |
| Substrate PubChem CID | 5316105 |
| Substrate Smiles | CC1=CCCC(=CC2C(C2(C)C)CCC(=CCC1)C)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 4-hydroxycasbene |
| Product Chemical Formula | C20H32O |
| Product PubChem CID | 154824095 |
| Product Smiles | C/C/1=CCC/C(=C/CC(/C(=C/[C@@H]2[C@@H](C2(C)C)CC1)/C)O)/C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Oxidation and cyclization of casbene in the biosynthesis of Euphorbia factors from mature seeds of Euphorbia lathyris L. |
| PMID: | 27506796 |
| Journal: | Proceedings of the National Academy of Sciences of the United States of America |
| Year: | 2016/8/23 |