Detail Information
P450 Protein:
| P450 Symbol | NfP450 |
| Protein Name | Sesterfisheric acid synthase (EC 1.-.-.-) (Cytochrome P450 monooxygenase NfP450) (Sesterfisheric acid biosynthesis cluster protein NfP450) |
| Uniprot ID | A1DN29 |
| EC Number | 1.-.-.- |
| Gene ID | 4584612 |
| Species | Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Txid | 331117 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C25H42O + C17H21N4O9P-2 + O2 → C25H39O3- + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 78407 |
+
+
NfP450
+
+
+
Substrate Information:
| Substrate Name | sesterfisherol |
| Substrate Chemical Formula | C25H42O |
| Substrate PubChem CID | 122209990 |
| Substrate Smiles | CC1CCC2C(CCC2=C(C3(C1CC4(CCC(C4C3)C(C)C)C)O)C)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | sesterfisherate |
| Product Chemical Formula | C25H39O3- |
| Product PubChem CID | 129320314 |
| Product Smiles | CC1CCC2C(CCC2=C(C3(C1CC4(CCC(C4C3)C(C)C)C(=O)[O-])O)C)C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Genomic islands in the pathogenic filamentous fungus Aspergillus fumigatus. |
| PMID: | 18404212 |
| Journal: | Scientific reports |
| Year: | 2018/2/6 |
| Title: | Genome Mining for Sesterterpenes Using Bifunctional Terpene Synthases Reveals a Unified Intermediate of Di/Sesterterpenes. |
| PMID: | 26332841 |
| Journal: | PLoS genetics |
| Year: | 2015/9/16 |
| Title: | Theoretical Study of Sesterfisherol Biosynthesis: Computational Prediction of Key Amino Acid Residue in Terpene Synthase. |
| PMID: | 29410538 |
| Journal: | Journal of the American Chemical Society |
| Year: | 2008/4/11 |