Detail Information
P450 Protein:
| P450 Symbol | otaC |
| Protein Name | Cytochrome P450 monooxygenase otaC (EC 1.14.14.-) (Ochratoxin biosynthesis cluster protein 3) (Ochratoxin biosynthesis cluster protein C) |
| Uniprot ID | A2R6G9 |
| EC Number | 1.14.14.- |
| Gene ID | 4988391 |
| Species | Aspergillus niger (strain ATCC MYA-4892 / CBS 513.88 / FGSC A1513) |
| Txid | 425011 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C11H12O3 + C17H21N4O9P-2 + O2 → C11H9O5- + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 72771 |
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otaC
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Substrate Information:
| Substrate Name | 7-methylmellein |
| Substrate Chemical Formula | C11H12O3 |
| Substrate PubChem CID | 10035470 |
| Substrate Smiles | CC1CC2=C(C(=C(C=C2)C)O)C(=O)O1 |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 7-carboxymellein |
| Product Chemical Formula | C11H9O5- |
| Product PubChem CID | 146036923 |
| Product Smiles | CC1CC2=C(C(=C(C=C2)C(=O)O)[O-])C(=O)O1 |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. |
| PMID: | 17259976 |
| Journal: | Journal of agricultural and food chemistry |
| Year: | 2012/4/16 |
| Title: | Strain-specific polyketide synthase genes of Aspergillus niger. |
| PMID: | 22341916 |
| Journal: | Toxins |
| Year: | 2020/1/ |
| Title: | Variation in Fumonisin and Ochratoxin Production Associated with Differences in Biosynthetic Gene Content in Aspergillus niger and A. welwitschiae Isolates from Multiple Crop and Geographic Origins. |
| PMID: | 27667988 |
| Journal: | Frontiers in microbiology |
| Year: | 2016/1/ |
| Title: | Comparative Genomic Analysis of Ochratoxin A Biosynthetic Cluster in Producing Fungi: New Evidence of a Cyclase Gene Involvement. |
| PMID: | 33391201 |
| Journal: | International journal of food microbiology |
| Year: | 2022/2/23 |
| Title: | Deletion and Overexpression of the AnOTAbzip Gene, a Positive Regulator of Ochratoxin A Biosynthesis in Aspergillus niger. |
| PMID: | 35143724 |
| Journal: | Nature biotechnology |
| Year: | 2022/8/12 |
| Title: | Insights into the Underlying Mechanism of Ochratoxin A Production in Aspergillus niger CBS 513.88 Using Different Carbon Sources. |
| PMID: | 36006213 |
| Journal: | |
| Year: | 2007/2/ |