Detail Information
P450 Protein:
| P450 Symbol | GAO |
| Protein Name | Germacrene A hydroxylase (EC 1.14.14.95) (Germacrene A oxidase) (HaGAO) |
| Uniprot ID | D5JBX0 |
| EC Number | 1.14.14.95 |
| Species | Helianthus annuus (Common sunflower) |
| Txid | 4232 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C15H24 + C17H21N4O9P-2 + O2 → C15H21O2- + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 30303 |
+
+
GAO
+
+
+
Substrate Information:
| Substrate Name | (+)-(R)-germacrene A |
| Substrate Chemical Formula | C15H24 |
| Substrate PubChem CID | 9548705 |
| Substrate Smiles | CC1=CCCC(=CCC(CC1)C(=C)C)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | germacra-1(10),4,11(13)-trien-12-oate |
| Product Chemical Formula | C15H21O2- |
| Product PubChem CID | 25245824 |
| Product Smiles | CC1=CCCC(=CCC(CC1)C(=C)C(=O)[O-])C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Biochemical conservation and evolution of germacrene A oxidase in asteraceae. |
| PMID: | 20351109 |
| Journal: | ACS chemical biology |
| Year: | 2018/6/15 |
| Title: | Biosynthesis of Eupatolide-A Metabolic Route for Sesquiterpene Lactone Formation Involving the P450 Enzyme CYP71DD6. |
| PMID: | 29758164 |
| Journal: | The Journal of biological chemistry |
| Year: | 2010/5/28 |
| Title: | Non-volatile natural products in plant glandular trichomes: chemistry, biological activities and biosynthesis. |
| PMID: | 30468448 |
| Journal: | Natural product reports |
| Year: | 2019/4/17 |