Detail Information
P450 Protein:
| P450 Symbol | mpaDE |
| Protein Name | Cytochrome P450 monooxygenase mpaDE (EC 1.-.-.-) (Mycophenolic acid biosynthesis cluster fusion protein DE) |
| Uniprot ID | I7LRH3 |
| EC Number | 1.-.-.- |
| Species | Penicillium brevicompactum |
| Txid | 5074 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C9H9O5- + H+ → C9H8O4 + H2O |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 66672 |
+
mpaDE
+
Substrate Information:
| Substrate Name | 4,6-dihydroxy-2-(hydroxymethyl)-3-methylbenzoate |
| Substrate Chemical Formula | C9H9O5- |
| Substrate PubChem CID | 155486812 |
| Substrate Smiles | CC1=C(C=C(C(=C1CO)C(=O)O)O)[O-] |
| Substrate Structure |
| Substrate Name | H+ |
| Substrate Chemical Formula | H+ |
| Substrate PubChem CID | 1038 |
| Substrate CAS Number | 12408-02-5 |
| Substrate Smiles | [H+] |
| Substrate Structure |
Product Information:
| Product Name | 5,7-dihydroxy-4-methylphthalide |
| Product Chemical Formula | C9H8O4 |
| Product PubChem CID | 14503052 |
| Product Smiles | CC1=C2COC(=O)C2=C(C=C1O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | Molecular basis for mycophenolic acid biosynthesis in Penicillium brevicompactum. |
| PMID: | 21398490 |
| Journal: | Chembiochem : a European journal of chemical biology |
| Year: | 2015/3/2 |
| Title: | Involvement of a natural fusion of a cytochrome P450 and a hydrolase in mycophenolic acid biosynthesis. |
| PMID: | 22544261 |
| Journal: | Applied and environmental microbiology |
| Year: | 2011/5/ |
| Title: | Functional characterization of MpaG', the O-methyltransferase involved in the biosynthesis of mycophenolic acid. |
| PMID: | 25630520 |
| Journal: | |
| Year: | 2012/7/ |