Detail Information

P450 Protein:

P450 SymbolGO
Protein NameGeissoschizine oxidase (SnvGO) (EC 1.14.19.80) (CYP450 monooxygenase GO) (Cytochrome P450 GO)
Uniprot IDP0DO79
EC Number1.14.19.80
SpeciesStrychnos nux-vomica (Poison nut) (Strychnine tree)
Txid 28545
P450 Protein Structure
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
Powered by PDBe Molstar & AlphaFold
Subcellular Location
Protein Sequence GO

Reaction Scheme:

Chemical Conversion C21H24N2O3 + C17H21N4O9P-2 + O2 → C21H23N2O3+ + C17H18N4O9P-3 + H2O
Reaction TypeOxidation
Chemical BondCH2;-N-
RheaID 58516

(19E)-geissoschizine

+

FMNH2

+

O2

GO

3,17-didehydrostemmadenine

+

FMN

+

H2O

Substrate Information:

Substrate Name (19E)-geissoschizine
Substrate Chemical FormulaC21H24N2O3
Substrate PubChem CID10948159
Substrate SmilesCC=C1CN2CCC3=C(C2CC1C(=CO)C(=O)OC)NC4=CC=CC=C34
Substrate Structure
Substrate Name FMNH2
Substrate Chemical FormulaC17H21N4O9P-2
Substrate PubChem CID44229161
Substrate SmilesCC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O
Substrate Structure
Substrate Name O2
Substrate Chemical FormulaO2
Substrate PubChem CID977
Substrate CAS Number7782-44-7
Substrate SmilesO=O
Substrate Structure

Product Information:

Product Name 3,17-didehydrostemmadenine
Product Chemical FormulaC21H23N2O3+
Product PubChem CID171904539
Product SmilesCC=C1C[N+]2=CCC1C(C3=C(CC2)C4=CC=CC=C4N3)(C=O)C(=O)OC
Product Structure
Product Name FMN
Product Chemical FormulaC17H18N4O9P-3
Product PubChem CID44229199
Product SmilesCC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O
Product Structure
Product Name H2O
Product Structure

References:

Title: Biosynthesis of strychnine.
PMID: 35794473
Journal: Nature
Year: 2022/7/

Contact zhy1001@alu.uestc.edu.cn or yangzhang@cdutcm.edu.cn
© Department of Bioinformatics