Detail Information
P450 Protein:
| P450 Symbol | fsoE |
| Protein Name | Cytochrome P450 monooxygenase fsoE (EC 1.-.-.-) (Fuscoatroside biosynthesis cluster protein E) |
| Uniprot ID | P9WEG9 |
| EC Number | 1.-.-.- |
| Species | Humicola fuscoatra |
| Txid | 112175 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C30H50O3 + C17H21N4O9P-2 + O2 → C30H48O3 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | |
| RheaID | 82767 |
+
+
fsoE
+
+
+
Substrate Information:
| Substrate Name | 2alpha,19beta-dihydroxyisomotiol |
| Substrate Chemical Formula | C30H50O3 |
| Substrate PubChem CID | 172429312 |
| Substrate Smiles | CC(C)C1CC(C2C1(CCC3(C2(CCC4=C3CCC5C4(CC(C(C5(C)C)O)O)C)C)C)C)O |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 2alpha-hydroxyismotiol-19-one |
| Product Chemical Formula | C30H48O3 |
| Product PubChem CID | 172429313 |
| Product Smiles | CC(C)C1CC(=O)C2C1(CCC3(C2(CCC4=C3CCC5C4(CC(C(C5(C)C)O)O)C)C)C)C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Biosynthesis of Enfumafungin-type Antibiotic Reveals an Unusual Enzymatic Fusion Pattern and Unprecedented C-C Bond Cleavage. |
| PMID: | 38654452 |
| Journal: | Journal of the American Chemical Society |
| Year: | 2024/5/8 |