Detail Information
P450 Protein:
| P450 Symbol | sttB |
| Protein Name | Cytochrome P450 monooxygenase sttB (EC 1.-.-.-) (Aspterpenacid biosynthesis cluster protein sttB) (Ophiobolin family sesterterpenoid biosynthesis cluster protein B) |
| Uniprot ID | Q0CRW7 |
| EC Number | 1.-.-.- |
| Gene ID | 4317925 |
| Species | Aspergillus terreus (strain NIH 2624 / FGSC A1156) |
| Txid | 341663 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C25H42O + C17H21N4O9P-2 + O2 → C25H42O2 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 71999 |
+
+
sttB
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+
+
Substrate Information:
| Substrate Name | preaspterpenacid acid I |
| Substrate Chemical Formula | C25H42O |
| Substrate PubChem CID | 163358938 |
| Substrate Smiles | CC1CCC23C(CCC2C3(C4(C1CC5(CCC(C5C4)C(C)C)C)O)C)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | preaspterpenacid acid II |
| Product Chemical Formula | C25H42O2 |
| Product PubChem CID | 163358949 |
| Product Smiles | CC1CCC23C(CCC2C3(C4(C1CC5(CCC(C5C4)C(C)C)C)O)CO)C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | A scalable platform to identify fungal secondary metabolites and their gene clusters. |
| PMID: | 28604695 |
| Journal: | Organic letters |
| Year: | 2017/8/ |
| Title: | Genome-Based Discovery of Enantiomeric Pentacyclic Sesterterpenes Catalyzed by Fungal Bifunctional Terpene Synthases. |
| PMID: | 34085529 |
| Journal: | Nature chemical biology |
| Year: | 2021/6/18 |