Detail Information
P450 Protein:
| P450 Symbol | CYP74A2 |
| Protein Name | Allene oxide synthase CYP74A2 (EC 4.2.1.92) (Cytochrome P450 74A2) (Hydroperoxide dehydratase) (Rubber particle protein) (RPP) |
| Uniprot ID | Q40778 |
| EC Number | 4.2.1.92 |
| Species | Parthenium argentatum (Guayule rubber plant) |
| Txid | 35935 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C18H31O4- → C18H29O3- + H2O |
| Reaction Type | Reduction |
| Chemical Bond | O-O |
| RheaID | 84075 |
CYP74A2
+
Substrate Information:
| Substrate Name | (13S)-hydroperoxy-(9Z,11E)-octadecadienoate |
| Substrate Chemical Formula | C18H31O4- |
| Substrate PubChem CID | 21158461 |
| Substrate Smiles | CCCCCC(C=CC=CCCCCCCCC(=O)[O-])OO |
| Substrate Structure |
Product Information:
| Product Name | (9Z,13S)-12,13-epoxyoctadeca-9,11-dienoate |
| Product Chemical Formula | C18H29O3- |
| Product PubChem CID | 46878374 |
| Product Smiles | CCCCCC1C(=CC=CCCCCCCCC(=O)[O-])O1 |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | The major protein of guayule rubber particles is a cytochrome P450. Characterization based on cDNA cloning and spectroscopic analysis of the solubilized enzyme and its reaction products. |
| PMID: | 7721745 |
| Journal: | Proceedings of the National Academy of Sciences of the United States of America |
| Year: | 1998/7/17 |
| Title: | Aspirin inhibition and acetylation of the plant cytochrome P450, allene oxide synthase, resembles that of animal prostaglandin endoperoxide H synthase. |
| PMID: | 9660772 |
| Journal: | The Journal of biological chemistry |
| Year: | 2008/9/16 |
| Title: | Modes of heme binding and substrate access for cytochrome P450 CYP74A revealed by crystal structures of allene oxide synthase. |
| PMID: | 18787124 |
| Journal: | |
| Year: | 1995/4/14 |