Detail Information
P450 Protein:
| P450 Symbol | polB |
| Protein Name | Cytochrome P450 monooxygenase polB (EC 1.-.-.-) (Polytolypin biosynthesis cluster protein B) |
| Uniprot ID | A0A2B7YD52 |
| EC Number | 1.-.-.- |
| Species | Polytolypa hystricis (strain UAMH7299) |
| Txid | 1447883 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C30H44O6-2 + C17H21N4O9P-2 + O2 → C30H44O7-2 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 80235 |
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polB
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Substrate Information:
| Substrate Name | 2-deoxypolytolypin |
| Substrate Chemical Formula | C30H44O6-2 |
| Substrate PubChem CID | 171392389 |
| Substrate Smiles | CC(C)C1CCC2C1(CCC3(C2(CCC4C3=CCC5C4(C(CC(C5(C)C(=O)[O-])O)O)C(=O)[O-])C)C)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | polytolypin |
| Product Chemical Formula | C30H44O7-2 |
| Product PubChem CID | 171392388 |
| Product Smiles | CC(C)[C@H]1CC[C@@H]2[C@@]1(CC[C@]3([C@]2(CC[C@H]4C3=CC[C@@H]5[C@@]4([C@@H]([C@H]([C@@H]([C@@]5(C)C(=O)[O-])O)O)O)C(=O)[O-])C)C)C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Genome analysis reveals evolutionary mechanisms of adaptation in systemic dimorphic fungi. |
| PMID: | 29540755 |
| Journal: | Scientific reports |
| Year: | 2023/1/25 |
| Title: | Biosynthetic characterization of the antifungal fernane-type triterpenoid polytolypin for generation of new analogues via combinatorial biosynthesis. |
| PMID: | 36602159 |
| Journal: | Organic & biomolecular chemistry |
| Year: | 2018/3/14 |