Detail Information
P450 Protein:
| P450 Symbol | FrzC |
| Protein Name | Cytochrome P450 monooxygenase FrzC (EC 1.14.14.-) (FR901483 biosynthesis cluster protein C) |
| Uniprot ID | A0A7T8F1I4 |
| EC Number | 1.14.14.- |
| Species | Cladobotryum sp |
| Txid | 2040732 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C18H23N2O2+ + C17H21N4O9P-2 + O2 → C18H21N2O2+ + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Redox |
| Chemical Bond | |
| RheaID | 83579 |
+
+
FrzC
+
+
+
Substrate Information:
| Substrate Name | (S,S)-2,5-di-(p-hydroxybenzyl)piperazine |
| Substrate Chemical Formula | C18H23N2O2+ |
| Substrate PubChem CID | 134820091 |
| Substrate Smiles | C1C(NCC([NH2+]1)CC2=CC=C(C=C2)O)CC3=CC=C(C=C3)O |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | (1S,4S)-4-[(4-hydroxyphenyl)methyl]-2,5-diazaspiro[bicyclo[3.2.1]octane-6,1′-cyclohexane]-2′,5′-dien-4′-one |
| Product Chemical Formula | C18H21N2O2+ |
| Product PubChem CID | 172879925 |
| Product Smiles | C1C2CN(C13C=CC(=O)C=C3)C(C[NH2+]2)CC4=CC=C(C=C4)O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Biosynthesis of the Immunosuppressant (-)-FR901483. |
| PMID: | 33372776 |
| Journal: | Journal of the American Chemical Society |
| Year: | 2021/1/13 |