Detail Information
P450 Protein:
| P450 Symbol | |
| Protein Name | Taxoid 2-alpha-hydroxylase (EC 1.14.14.-) |
| Uniprot ID | Q6JD68 |
| EC Number | 1.14.14.- |
| Species | Taxus canadensis (Canadian yew) |
| Txid | 88032 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C28H40O9 + C17H21N4O9P-2 + O2 → C28H40O10 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| RheaID | 71407 |
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Substrate Information:
| Substrate Name | 7beta-hydroxytaxusin |
| Substrate Chemical Formula | C28H40O9 |
| Substrate PubChem CID | 56927995 |
| Substrate Smiles | CC1=C2C(C(C3(C(CC(C2(C)C)CC1OC(=O)C)C(=C)C(CC3O)OC(=O)C)C)OC(=O)C)OC(=O)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 2alpha,7beta-dihydroxytaxusin |
| Product Chemical Formula | C28H40O10 |
| Product PubChem CID | 163358868 |
| Product Smiles | CC1=C2C(C(C3(C(CC(C(=C)C3C(C(C2(C)C)CC1OC(=O)C)O)OC(=O)C)O)C)OC(=O)C)OC(=O)C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Molecular cloning and characterization of a cytochrome P450 taxoid 2alpha-hydroxylase involved in Taxol biosynthesis. |
| PMID: | 15178487 |
| Journal: | Archives of biochemistry and biophysics |
| Year: | 2004/7/1 |